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human cd4 t lymphocyte line jurkat  (ATCC)


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    ATCC human cd4 t lymphocyte line jurkat
    Human Cd4 T Lymphocyte Line Jurkat, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4475 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cd4+t+lymphocyte+line+jurkat/Jurkat%2C+Clone+E6-1/pm41322423-48-1-13
    Average 99 stars, based on 4475 article reviews
    human cd4 t lymphocyte line jurkat - by Bioz Stars, 2026-10
    99/100 stars

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    Cell Culture:

    Article Title: Inflammatory and redox reprogramming of macrophages by HIV cell-to-cell transmission inhibits bone resorption capacity.
    Article Snippet: Then, for osteoclast differentiation, Mf were further cultured in a-MEM medium (Gibco; cat. 11900073) supplemented with 10% FBS, 2 mM of L‐glutamine (Gibco; cat. 25030081), 1 mM of sodium pyruvate (Gibco; cat. 11360070), and penicillin‐streptomycin (Gibco; cat. 15140122) (a‐MEM complete medium), M-CSF (10 ng/mL; StemCell Technologies; cat. 78150), and RANKL (30 ng/mL; StemCell Technologies; cat. 78214) for 9 additional days (mature osteoclasts). .. The human CD4+ T lymphocyte line Jurkat (Jk; clone E6.1), obtained from the American Type Culture Collection (ATCC, USA), was cultured in RPMI complete medium and maintained at 37°C in a humidified 5% CO2 incubator. .. The cells were routinely tested for mycoplasma contamination using the MycoAlert Mycoplasma Detection Kit (Lonza, Tampa, FL, USA; LT07-318).

    Article Title: Inflammatory and redox reprogramming of macrophages by HIV cell-to-cell transmission inhibits bone resorption capacity
    Article Snippet: Then, for osteoclast differentiation, Mf were further cultured in α-MEM medium (Gibco; cat. 11900073) supplemented with 10% FBS, 2 mM of L‐glutamine (Gibco; cat. 25030081), 1 mM of sodium pyruvate (Gibco; cat. 11360070), and penicillin‐streptomycin (Gibco; cat. 15140122) (α‐MEM complete medium), M-CSF (10 ng/mL; StemCell Technologies; cat. 78150), and RANKL (30 ng/mL; StemCell Technologies; cat. 78214) for 9 additional days (mature osteoclasts). .. The human CD4 + T lymphocyte line Jurkat (Jk; clone E6.1), obtained from the American Type Culture Collection (ATCC, USA), was cultured in RPMI complete medium and maintained at 37°C in a humidified 5% CO 2 incubator. .. The cells were routinely tested for mycoplasma contamination using the MycoAlert ® Mycoplasma Detection Kit (Lonza, Tampa, FL, USA; LT07-318).

    Incubator:

    Article Title: Inflammatory and redox reprogramming of macrophages by HIV cell-to-cell transmission inhibits bone resorption capacity.
    Article Snippet: Then, for osteoclast differentiation, Mf were further cultured in a-MEM medium (Gibco; cat. 11900073) supplemented with 10% FBS, 2 mM of L‐glutamine (Gibco; cat. 25030081), 1 mM of sodium pyruvate (Gibco; cat. 11360070), and penicillin‐streptomycin (Gibco; cat. 15140122) (a‐MEM complete medium), M-CSF (10 ng/mL; StemCell Technologies; cat. 78150), and RANKL (30 ng/mL; StemCell Technologies; cat. 78214) for 9 additional days (mature osteoclasts). .. The human CD4+ T lymphocyte line Jurkat (Jk; clone E6.1), obtained from the American Type Culture Collection (ATCC, USA), was cultured in RPMI complete medium and maintained at 37°C in a humidified 5% CO2 incubator. .. The cells were routinely tested for mycoplasma contamination using the MycoAlert Mycoplasma Detection Kit (Lonza, Tampa, FL, USA; LT07-318).



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    FIG. 2. Proliferation of <t>CD4</t> <t>T</t> <t>lymphocytes</t> in response to CEACAM1 ligation by neisserial OMVs or antibody. Primary CD4 T cells were cultured in the presence of IL-2 and cross-linked CD3ε- and CD28-specific antibodies with various protein concentrations of either Nl-OMVs (gray bars) or Nm-OMVs (black bars) (A) or CEACAM-specific antisera (black bars) or nonreactive isotype control antibodies (gray bars) or no additions (white bars) (B). The calculated increase in culture density is relative to the number of cells present at the onset of the experiment (time 0 h). In each instance, error bars indicate the standard deviations based upon values from six replicate samples, with results being representative of three independent experiments. Asterisks indicate P values of 0.01 for comparison with Nl-OMVs (A) or isotype control antibodies (B).
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    FIG. 2. Proliferation of CD4 T lymphocytes in response to CEACAM1 ligation by neisserial OMVs or antibody. Primary CD4 T cells were cultured in the presence of IL-2 and cross-linked CD3ε- and CD28-specific antibodies with various protein concentrations of either Nl-OMVs (gray bars) or Nm-OMVs (black bars) (A) or CEACAM-specific antisera (black bars) or nonreactive isotype control antibodies (gray bars) or no additions (white bars) (B). The calculated increase in culture density is relative to the number of cells present at the onset of the experiment (time 0 h). In each instance, error bars indicate the standard deviations based upon values from six replicate samples, with results being representative of three independent experiments. Asterisks indicate P values of 0.01 for comparison with Nl-OMVs (A) or isotype control antibodies (B).

    Journal: Infection and Immunity

    Article Title: Neisserial Outer Membrane Vesicles Bind the Coinhibitory Receptor Carcinoembryonic Antigen-Related Cellular Adhesion Molecule 1 and Suppress CD4 + T Lymphocyte Function

    doi: 10.1128/iai.00222-07

    Figure Lengend Snippet: FIG. 2. Proliferation of CD4 T lymphocytes in response to CEACAM1 ligation by neisserial OMVs or antibody. Primary CD4 T cells were cultured in the presence of IL-2 and cross-linked CD3ε- and CD28-specific antibodies with various protein concentrations of either Nl-OMVs (gray bars) or Nm-OMVs (black bars) (A) or CEACAM-specific antisera (black bars) or nonreactive isotype control antibodies (gray bars) or no additions (white bars) (B). The calculated increase in culture density is relative to the number of cells present at the onset of the experiment (time 0 h). In each instance, error bars indicate the standard deviations based upon values from six replicate samples, with results being representative of three independent experiments. Asterisks indicate P values of 0.01 for comparison with Nl-OMVs (A) or isotype control antibodies (B).

    Article Snippet: Purified primary lymphocytes and the Jurkat CD4 human T-lymphocyte line (ATCC CRL-10915) were both maintained in RPMI 1640 medium (Invitrogen Life Technologies, Burlington, Ontario) supplemented with 10% heat-inactivated fetal bovine serum and 4 mM GlutaMAX (Invitrogen), referred to as RPMI-G.

    Techniques: Ligation, Cell Culture, Control, Comparison

    FIG. 3. Proliferation and CD69 expression of CD4 T lymphocytes in response to CEACAM1 binding by Opa-specific neisserial OMVs. Jurkat CD4 T cells were cultured in the presence of IL-2 and/or cross-linked CD3ε-specific antibodies with OMVs (50 g/ml protein) prepared from N. gonorrhoeae-expressing defined Opa variants. OMVs that bind CEACAM1 are indicated with black bars. (A) The mean increase in relative culture density was calculated relative to the number of cells in uninfected samples, which was defined as 100%. Error bars indicate the standard deviations based upon values from six quadrants counted for each sample. (B) The proportion of cells expressing the early activation marker CD69 16 h following the onset of the experiment was determined by flow cytometry, with standard deviation calculations based upon three independent samples. Asterisks indicate P values of 0.0002 (A) or 0.005 (B) for comparison with the other OMV-treated samples in each set.

    Journal: Infection and Immunity

    Article Title: Neisserial Outer Membrane Vesicles Bind the Coinhibitory Receptor Carcinoembryonic Antigen-Related Cellular Adhesion Molecule 1 and Suppress CD4 + T Lymphocyte Function

    doi: 10.1128/iai.00222-07

    Figure Lengend Snippet: FIG. 3. Proliferation and CD69 expression of CD4 T lymphocytes in response to CEACAM1 binding by Opa-specific neisserial OMVs. Jurkat CD4 T cells were cultured in the presence of IL-2 and/or cross-linked CD3ε-specific antibodies with OMVs (50 g/ml protein) prepared from N. gonorrhoeae-expressing defined Opa variants. OMVs that bind CEACAM1 are indicated with black bars. (A) The mean increase in relative culture density was calculated relative to the number of cells in uninfected samples, which was defined as 100%. Error bars indicate the standard deviations based upon values from six quadrants counted for each sample. (B) The proportion of cells expressing the early activation marker CD69 16 h following the onset of the experiment was determined by flow cytometry, with standard deviation calculations based upon three independent samples. Asterisks indicate P values of 0.0002 (A) or 0.005 (B) for comparison with the other OMV-treated samples in each set.

    Article Snippet: Purified primary lymphocytes and the Jurkat CD4 human T-lymphocyte line (ATCC CRL-10915) were both maintained in RPMI 1640 medium (Invitrogen Life Technologies, Burlington, Ontario) supplemented with 10% heat-inactivated fetal bovine serum and 4 mM GlutaMAX (Invitrogen), referred to as RPMI-G.

    Techniques: Expressing, Binding Assay, Cell Culture, Activation Assay, Marker, Cytometry, Standard Deviation, Comparison